Chinese | English
用户名 密码 验证码 验证码
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO16
Recent progress of this line F1 individuals have been identified
Gene name rasa3
Ensembl ID ENSDARG00000063371
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
5’- GGAGAATCTGGACACAAACA ggg -3’
Cas9 target site 2 sequence
Position in the gene Exon 13
Restriction enzyme (RE) T7EI
PCR primer (forward) 5’- TGTGCATACACCCTATTGGTTAC -3’
PCR primer (reverse) 5’- CTCATTTCATTTGTCTTTTGTACTG -3’
Length of expected PCR product (bp) 500
Length of expected RE digestion product (bp) 330,270
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 16
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 60
Survival rate of F0 embryo @ 1dpf (%) 80
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO16.pdf
Mutation efficiency in F0 embryos (%) 60
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 20%
Wild-type (WT) sequence tcggagaatctggacaca a acagggaaaacctgcg
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 tcggagaatctggacacaa- - - -ggaaaacctgcg (-4 bp)
F0-2 tcggagaatctggacacaCaGacagggaaaacctgcg (+2 bp)
Allele name and predicted product 16a
16b
tcggagaatctggacacaa- - - -ggaaaacctgcg; Truncated protein (437 aa)
tcggagaatctggacacaCaGacagggaaaacctgcg; Truncated protein (439 aa)
突变创建时间
填表时间 2013/12/24
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2016 国家斑马鱼资源中心 版权所有