Chinese | English
用户名 密码 验证码 验证码
如有订购ZKO项目创建品系需求,请点击ZKO品系购买进行下单,如有其它问题请参见ZKO订购说明。

ZKO number ZKO10
Recent progress of this line F2 individuals have been indentified
Gene name tmco3
Ensembl ID ENSDARG00000075108
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCAGAGAAACACCAGCAGG agg
Cas9 target site 2 sequence
Position in the gene Exon 2
Restriction enzyme (RE) T7E1
PCR primer (forward) TGGTTGGCCGCTAGTGTATTTT
PCR primer (reverse) TTGTGTTAGTGCACCTGTTTG
Length of expected PCR product (bp) 400
Length of expected RE digestion product (bp) 255+140
Plasmids used for making Cas9 mRNA pSP6-2sNLS-spCas9
Plasmids?used?for?making?gRNA pT7-gRNA-ZKOpT7-gRNA-ZKO10
Primers?used?for?making?gRNA Pr-ZKO 10
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 50
Survival rate of F0 embryo @ 1dpf (%) 91.60
Method to estimate mutation efficiency T7EI detection
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO10.pdf
Mutation efficiency in F0 embryos (%) 20
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 71% (10/14: Number of positive F0 / Total number of F0 screened)
Wild-type (WT) sequence TGGTGGCGGCAGAGAAACACCAGCAGGAGGCG
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1 TGGTGGCGGCAGAGAAACACCAGCTGAAAGTGAAAAGAGG (+10 bp)
F0-2 TGGTGGCGGCAG--------------GAGGCG (-14 bp)
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 TGGTGGCGGCAGAGAAACACCAGCTGAAAGTGAAAAGAGG (+10 bp)
F0-2 TGGTGGCGGCAG--------------GAGGCG (-14 bp)
Allele name and predicted product 10a
10b
GGCAGAGAAACACCAGCTGAAAGTGA Truncated protein (446aa)
TGGTGGCGGCAG--------------GAGGCGTruncated protein (446 aa)
突变创建时间 2014-03-12
填表时间 2013/12/24
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotype Information:
友情链接: 更多
地址:武汉市东湖南路7号中科院水生所; 电话:027-68780570; 网址:http://www.zfish.cn/; 邮箱:zebrafish@ihb.ac.cn
Copyright © 2012 - 2016 国家斑马鱼资源中心 版权所有