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ZKO number ZKO13
Recent progress of this line F2 individuals have been indentified
Gene name cenpe
Ensembl ID ENSDARG00000063385
Zebrafish strain AB
Cas9 target site 1 sequence sense strand
GGCATCTGAGAGCTCGGAGCcgg
Cas9 target site 2 sequence
Position in the gene exon 2
Restriction enzyme (RE) Sac I(GAGCT|C)
PCR primer (forward) TACTATTTTATGTTCCTGTTATGG
PCR primer (reverse) GCTCTACTACTTGGATTGCC
Length of expected PCR product (bp) 401
Length of expected RE digestion product (bp) 175+102+124
Plasmids used for making Cas9 mRNA pXT7-hCAS9
Plasmids?used?for?making?gRNA pMD19T-gRNA
Primers?used?for?making?gRNA Pr-ZKO 13
Dosage of injected Cas9 mRNA (ng/ul) 300
Dosage of injected gRNA (ng/ul) 30
Survival rate of F0 embryo @ 1dpf (%) 95
Method to estimate mutation efficiency Restriction enzyme digestionRE digestion (Sac I)
Stage of the embryos collected for mutation detection http://www.zfish.cn/upload/file/ZKOPDF/ZKO13.pdf
Mutation efficiency in F0 embryos (%) 30
Picture?from?F0?embryos
? (gel?picture?or?sequencing?picture)
Mutation type Indel
Germline transmission efficiency in adult F0 35%(6/17)
Wild-type (WT) sequence GGCATCTGAGAGCTCGGAGC
Germline mutations in selected F0
 (By screening F1 embryos)
F0-1
F0-2
Mosaicism in selected F0
 (By screening F1 adults)
F0-1 GGCATCTGA-------GAGC(-7bp)
F0-2
Allele name and predicted product 13a
13b
GGCATCTGA-------GAGC; Truncated protein(98aa)
突变创建时间 2014-04-10
填表时间 2013/12/18
修改时间 2016/8/8
PI/负责人 (工作单位)
Genotype Information:
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